A SOS BOX PATTERN FOR LEPTOSPIRA SPP

• DOI: 10.22533/at.ed.3771916011

• Palavras-chave: Atena

• Keywords: genomics, SOS response, Leptospira spp

• Abstract:

  Leptospirosis is an important

  zoonosis classified as a neglected tropical

  disease, responsible for serious public health

  problems resulting in costs to the economy. The

  disease is caused by pathogenic bacteria of

  the Leptospira genus which comprehends both

  pathogenic species and saprophytic ones. The

  SOS response is a bacteria defense mechanism

  against DNA damage caused by ultraviolet

  radiation, high concentrations of O2

   and Fe

  inside the cell and antibiotics. This system is

  well studied in Escherichia coli where more than

  40 genes, including recA, dinP, uvrA and recN

  genes, were found to be involved in this kind

  of response and regulated by lexA and recA

  regulators. In Leptospira spp this response is

  not well characterized, especially regarding the

  saprophytic species. This study characterized

  a SOS box motif in three leptospira species

  using genes regulated by lexA previously

  studied in Leptospira interrogans with ChIP-Seq

  experiment (chromosome immunoprecipitation

  coupled with massive parallel sequencing

  experiment) when exponential culture of

  bacteria were irradiated (IR) or not (NI) to a

  UV-C germicidal lamp (254nm), to a dose known

  to kill 50 - 60\% of cells. MEME generated the

  SOS box motif that was subsequently used

  within MAST to search for this pattern in the

  genomes of Leptospira borgpetersenii serovar

  serovar bovis, Leptospira interrogans serovar

  Copenhageni Fiocruz and Leptospira biflexa

  serovar Patoc Patoc Ames 1. The SOS box

  motif retrieved genes known to be regulated

  by lexA, therefore indicating that the SOS box

  identified can be used to identified other genes

  involved in the SOS response.

• Número de páginas: 15