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Corynebacterium pseudotuberculosis protein extract induces IP10 production in blood samples of individuals with pulmonary tuberculosis

Mycobacterium tuberculosis and Corynebacterium pseudotuberculosis are part of the CMRN group and share similarities among some virulence/pathogenicity factors. The objective of the present study was to use semi-purified extracts of C. pseudotuberculosis strains to evaluate antigenicity in samples of individuals diagnosed with active pulmonary tuberculosis. Voluntary participants with  positive tuberculosis (TB) diagnosis and treatment-naïve tuberculosis (n=28), without complaints, no history or symptomatology of TB or M. tuberculosis infection (n=28). With heparinized blood, cell stimulation was carried out using mitogen (PWM) and C. pseudotuberculosis protein extract (PAT-10, VD57 and FRC-41 strains). For cytokines measurement, BD Cytometric Bead Array (CBA) was used. Semi-purified extracts of all three C. pseudotuberculosis strains induced production of cytokines in blood samples from studied groups. In addition, they differentiated studied groups when compared to IFN-γ and IP-10. C. pseudotuberculosis strains extracts PAT10, VD57 and FRC41 induced production of IFN-γ, IP-10 in blood samples from individuals infected with M. tuberculosis. Under stimuli of extracts used, the detection of IFN-γ and IP-10 differentiated  the control group and active TB groups.

Corynebacterium pseudotuberculosis protein extract induces IP10 production in blood samples of individuals with pulmonary tuberculosis

DOI: 10.22533/at.ed.4352001075

Palavras chave: CORYNEBACTERIUM PSEUDOTUBERCULOSIS; CYTOKINES; MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSIS; ANTIGENICITY

Keywords: CORYNEBACTERIUM PSEUDOTUBERCULOSIS; CYTOKINES; MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSIS; ANTIGENICITY

Abstract:

Mycobacterium tuberculosis and Corynebacterium pseudotuberculosis are part of the CMRN group and share similarities among some virulence/pathogenicity factors. The objective of the present study was to use semi-purified extracts of C. pseudotuberculosis strains to evaluate antigenicity in samples of individuals diagnosed with active pulmonary tuberculosis. Voluntary participants with  positive tuberculosis (TB) diagnosis and treatment-naïve tuberculosis (n=28), without complaints, no history or symptomatology of TB or M. tuberculosis infection (n=28). With heparinized blood, cell stimulation was carried out using mitogen (PWM) and C. pseudotuberculosis protein extract (PAT-10, VD57 and FRC-41 strains). For cytokines measurement, BD Cytometric Bead Array (CBA) was used. Semi-purified extracts of all three C. pseudotuberculosis strains induced production of cytokines in blood samples from studied groups. In addition, they differentiated studied groups when compared to IFN-γ and IP-10. C. pseudotuberculosis strains extracts PAT10, VD57 and FRC41 induced production of IFN-γ, IP-10 in blood samples from individuals infected with M. tuberculosis. Under stimuli of extracts used, the detection of IFN-γ and IP-10 differentiated  the control group and active TB groups.

Autores

  • Caio Lopes Borges Andrade
  • Fúlvia Soares Campos de Sousa
  • Lilia Ferreira de Moura-Costa
  • Marcos Borges Ribeiro
  • Ramon Mendes dos Santos
  • Roberto Meyer
  • ROGERIO REIS CONCEICAO
  • Samanta Queiroz dos Santos
  • Silvânia Maria Andrade Cerqueira
  • Songelí Menezes Freire
  • Soraya Castro Trindade
  • Zunara Victória Santana Batista